हिंदी

Biotechnological Application in Medicine

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Estimated time: 3 minutes
CBSE: Class 12

Key Points: Biotechnological Application in Medicine

  • Genetically Engineered Insulin - Eli Lilly (1983) introduced Chain A & B DNA separately into E. coli plasmids, then combined them to form human insulin.
  • Why Recombinant Insulin - Animal insulin causes immune reactions; recombinant insulin is identical to human insulin, avoiding this.
  • Pro-insulin to Insulin - Pro-insulin has an extra C-peptide, which is removed during maturation to form functional insulin.
  • Gene Therapy - Functional genes are inserted into patients' cells to correct hereditary diseases; first used in 1990 for ADA deficiency.
  • PCR - Amplifies pathogen DNA to detect diseases like HIV even before symptoms appear.
  • ELISA - Detects infection using antigen-antibody interaction.
  • Molecular Diagnosis - PCR, ELISA & rDNA technology allow early disease detection where conventional methods fail.
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