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Methods of Separation - Chromatography Method

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notes

Chromatography Method:

Applications: 

  • To separate colours in a dye.
  • To separate pigments from natural colours
  • To separate drugs from the blood.

experiment

The experiment of Separation of dyes in black ink using chromatography.

Aim: To separate different colors from the ink with the help of chromatography.

Requirements: Filter paper, ink, jar, water, pencils, and scales.

Principle: An ink dye consists of several colors. These different dyes have different solubility. Dye with higher solubility in water dissolves quickly and move fast with the water and low soluble will move slowly separating from each other.

Procedure:

  • Take a thin strip of filter paper

  • Draw a line on it using a pencil, approximately 3 cm above the lower edge

  • Put a small drop of ink at the centre of the line. Let it dry.

  • Lower the filter paper into a jar containing water so that the drop of ink on the paper is just above the water level and leave it undisturbed.

  • Watch carefully, as the water rises up on the filter paper. Record your observations.

Observation:

  1. Filter paper as the water rises on it, It takes the more soluble colors within the top and less soluble colors are carried up later.

  2. We get different colours on filter paper by chromatography

  3. In the process of chromatography, the coloured spot rises on the paper strip as it is soluble in water and thus due to their solubility the spot rises up.

Inference/ Result: Components of a mixture have varying solubility, can be separated using chromatography.

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