Advertisements
Advertisements
Question
Compare TLC and Paper Chromatography techniques.
Advertisements
Solution
| Chromatography technique | TLC | Paper chromatography |
| Principle | It is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to a different extent. | It is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases. |
| Stationary phase | Solid (adsorbent like silica gel or alumina over a glass plate) | Liquid (water trapped in the fibres of a paper) |
| Mobile phase | Liquid (single solvent/mixture of solvents) | Liquid (single solvent/mixture of solvents) |
| Visualization of components of a mixture | Similar to TLC the coloured components are visible as coloured spots and the colourless components are observed under UV light or using a spraying agent. | |
APPEARS IN
RELATED QUESTIONS
Which separation technique will you apply for the separation of the following?
Different pigments from an extract of flower petals.
Give a brief description of the principle of the following technique taking an example.
Chromatography
Explain the principle of paper chromatography.
What are the advantages of chromatography?
Name the simplest type of chromatography?
What is meant by stationary phase and mobile phase in chromatography?
Select the correct alternative from the choices given for the following statement:
In chromatography the filter paper is
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
Name the different types of Chromatography and explain the principles underlying them.
Label the diagram and explain the process in your words.
The principle involved in paper chromatography is ______.
Paper chromatography is ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
In chromatography, which of the following statement is INCORRECT for Rf?
Complex mixtures are separated by ______.
In chromatography, the mobile phase is usually ______.
Who first described the method of chromatography?
