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प्रश्न
Compare TLC and Paper Chromatography techniques.
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उत्तर
| Chromatography technique | TLC | Paper chromatography |
| Principle | It is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to a different extent. | It is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases. |
| Stationary phase | Solid (adsorbent like silica gel or alumina over a glass plate) | Liquid (water trapped in the fibres of a paper) |
| Mobile phase | Liquid (single solvent/mixture of solvents) | Liquid (single solvent/mixture of solvents) |
| Visualization of components of a mixture | Similar to TLC the coloured components are visible as coloured spots and the colourless components are observed under UV light or using a spraying agent. | |
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संबंधित प्रश्न
Which separation technique will you apply for the separation of the following?
Different pigments from an extract of flower petals.
Explain the principle of paper chromatography.
What is chromatography?
What are the advantages of chromatography?
Name the simplest type of chromatography?
On what principle is Chromatography method based?
What is meant by stationary phase and mobile phase in chromatography?
Select the correct alternative from the choices given for the following statement:
In chromatography the filter paper is
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Name the different types of Chromatography and explain the principles underlying them.
Label the diagram and explain the process in your words.
Chromatography is based on the principle of ______.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
The principle involved in paper chromatography is ______.
Paper chromatography is ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
In chromatography, which of the following statement is INCORRECT for Rf?
In chromatography, the mobile phase is usually ______.
Who first described the method of chromatography?
