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प्रश्न
Compare TLC and Paper Chromatography techniques.
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उत्तर
| Chromatography technique | TLC | Paper chromatography |
| Principle | It is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to a different extent. | It is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases. |
| Stationary phase | Solid (adsorbent like silica gel or alumina over a glass plate) | Liquid (water trapped in the fibres of a paper) |
| Mobile phase | Liquid (single solvent/mixture of solvents) | Liquid (single solvent/mixture of solvents) |
| Visualization of components of a mixture | Similar to TLC the coloured components are visible as coloured spots and the colourless components are observed under UV light or using a spraying agent. | |
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संबंधित प्रश्न
Give a brief description of the principle of the following technique taking an example.
Chromatography
What is chromatography?
What are the advantages of chromatography?
Name the simplest type of chromatography?
Name the different types of chromatographic techniques.
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Define the Stationary phase.
Name the different types of Chromatography and explain the principles underlying them.
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
Label the diagram and explain the process in your words.
Chromatography is based on the principle of ______.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
The principle involved in paper chromatography is ______.
Paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
In chromatography, which of the following statement is INCORRECT for Rf?
Which basic principle is used in chromatography?
In chromatography, the mobile phase is usually ______.
Who first described the method of chromatography?
