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प्रश्न
Compare TLC and Paper Chromatography techniques.
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उत्तर
| Chromatography technique | TLC | Paper chromatography |
| Principle | It is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to a different extent. | It is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases. |
| Stationary phase | Solid (adsorbent like silica gel or alumina over a glass plate) | Liquid (water trapped in the fibres of a paper) |
| Mobile phase | Liquid (single solvent/mixture of solvents) | Liquid (single solvent/mixture of solvents) |
| Visualization of components of a mixture | Similar to TLC the coloured components are visible as coloured spots and the colourless components are observed under UV light or using a spraying agent. | |
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संबंधित प्रश्न
Explain the principle of paper chromatography.
What is chromatography?
What are the advantages of chromatography?
On what principle is Chromatography method based?
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
Label the diagram and explain the process in your words.
Chromatography is based on the principle of ______.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
The principle involved in paper chromatography is ______.
What is paper chromatography?
Paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
Complex mixtures are separated by ______.
Which basic principle is used in chromatography?
What is the function of the stationary phase in chromatography?
In chromatography, the mobile phase is usually ______.
Who first described the method of chromatography?
