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प्रश्न
Compare TLC and Paper Chromatography techniques.
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उत्तर
| Chromatography technique | TLC | Paper chromatography |
| Principle | It is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to a different extent. | It is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases. |
| Stationary phase | Solid (adsorbent like silica gel or alumina over a glass plate) | Liquid (water trapped in the fibres of a paper) |
| Mobile phase | Liquid (single solvent/mixture of solvents) | Liquid (single solvent/mixture of solvents) |
| Visualization of components of a mixture | Similar to TLC the coloured components are visible as coloured spots and the colourless components are observed under UV light or using a spraying agent. | |
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संबंधित प्रश्न
Which separation technique will you apply for the separation of the following?
Different pigments from an extract of flower petals.
Give a brief description of the principle of the following technique taking an example.
Chromatography
The best and latest technique for isolation, purification and separation of organic compounds is ______.
What is chromatography?
Name the simplest type of chromatography?
On what principle is Chromatography method based?
Select the correct alternative from the choices given for the following statement:
In chromatography the filter paper is
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Give names of two materials used as stationary phase in chromatography.
Name the different types of Chromatography and explain the principles underlying them.
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
Chromatography is based on the principle of ______.
The principle involved in paper chromatography is ______.
What is paper chromatography?
Paper chromatography is ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
In chromatography, which of the following statement is INCORRECT for Rf?
