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प्रश्न
Name the different types of Chromatography and explain the principles underlying them.
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उत्तर
Depending on the nature of the stationary phase i.e., whether it is a solid or a liquid, chromatography is classified into adsorption chromatography and partition chromatography.
- Adsorption chromatography: This technique is based on the principle of differential adsorption. Different solutes are adsorbed on an adsorbent to a different extent.
Adsorption chromatography is further classified into two types:
a. Column chromatography
b. Thin-layer chromatography - Partition chromatography: This technique is based on the continuous differential partitioning of components of a mixture between stationary and mobile phases. For example, paper chromatography.
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संबंधित प्रश्न
Explain the principle of paper chromatography.
The best and latest technique for isolation, purification and separation of organic compounds is ______.
What are the advantages of chromatography?
On what principle is Chromatography method based?
What is meant by stationary phase and mobile phase in chromatography?
Select the correct alternative from the choices given for the following statement:
In chromatography the filter paper is
Name the different types of chromatographic techniques.
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
Label the diagram and explain the process in your words.
Chromatography is based on the principle of ______.
The principle involved in paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
In chromatography, which of the following statement is INCORRECT for Rf?
Complex mixtures are separated by ______.
Who first described the method of chromatography?
