Advertisements
Advertisements
प्रश्न
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
Advertisements
उत्तर
- Thin-layer chromatography (TLC): If components are colourless but have the property of fluorescence then they can be visualized under UV light, or the plate can be kept in a chamber containing a few iodine crystals. The iodine vapours are adsorbed by the components and the spots appear brown. Also, a spraying agent like ninhydrin can also be used (for amino acids).
- Paper Chromatography: The spots of the separated colourless components may be observed either under ultra-violet light or by the use of an appropriate spraying agent.
APPEARS IN
संबंधित प्रश्न
Give a brief description of the principle of the following technique taking an example.
Chromatography
Explain the principle of paper chromatography.
The best and latest technique for isolation, purification and separation of organic compounds is ______.
Name the simplest type of chromatography?
On what principle is Chromatography method based?
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Define the Stationary phase.
Compare TLC and Paper Chromatography techniques.
Label the diagram and explain the process in your words.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
What is paper chromatography?
In chromatography technique, the purification of compound is independent of ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
Complex mixtures are separated by ______.
Which basic principle is used in chromatography?
What is the function of the stationary phase in chromatography?
In chromatography, the mobile phase is usually ______.
Who first described the method of chromatography?
