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प्रश्न
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
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उत्तर
- Thin-layer chromatography (TLC): If components are colourless but have the property of fluorescence then they can be visualized under UV light, or the plate can be kept in a chamber containing a few iodine crystals. The iodine vapours are adsorbed by the components and the spots appear brown. Also, a spraying agent like ninhydrin can also be used (for amino acids).
- Paper Chromatography: The spots of the separated colourless components may be observed either under ultra-violet light or by the use of an appropriate spraying agent.
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संबंधित प्रश्न
Give a brief description of the principle of the following technique taking an example.
Chromatography
Explain the principle of paper chromatography.
What are the advantages of chromatography?
On what principle is Chromatography method based?
What is meant by stationary phase and mobile phase in chromatography?
Name the different types of chromatographic techniques.
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Give names of two materials used as stationary phase in chromatography.
Name the different types of Chromatography and explain the principles underlying them.
Label the diagram and explain the process in your words.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
The principle involved in paper chromatography is ______.
What is paper chromatography?
In chromatography technique, the purification of compound is independent of ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
