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प्रश्न
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
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उत्तर
- Thin-layer chromatography (TLC): If components are colourless but have the property of fluorescence then they can be visualized under UV light, or the plate can be kept in a chamber containing a few iodine crystals. The iodine vapours are adsorbed by the components and the spots appear brown. Also, a spraying agent like ninhydrin can also be used (for amino acids).
- Paper Chromatography: The spots of the separated colourless components may be observed either under ultra-violet light or by the use of an appropriate spraying agent.
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संबंधित प्रश्न
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Explain the principle of paper chromatography.
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Select the correct alternative from the choices given for the following statement:
In chromatography the filter paper is
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In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
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- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
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Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
Compare TLC and Paper Chromatography techniques.
Chromatography is based on the principle of ______.
The principle involved in paper chromatography is ______.
What is paper chromatography?
Paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
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In chromatography, which of the following statement is INCORRECT for Rf?
Complex mixtures are separated by ______.
Which basic principle is used in chromatography?
Who first described the method of chromatography?
