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प्रश्न
Give a brief description of the principle of the following technique taking an example.
Chromatography
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उत्तर
Chromatography is one of the most useful methods for the separation and purification of organic compounds.
Principle:
It is based on the difference in movement of individual components of a mixture through the stationary phase under the influence of mobile phase.
For example, a mixture of red and blue ink can be separated by chromatography. A drop of the mixture is placed on the chromatogram. The component of the ink, which is less adsorbed on the chromatogram, moves with the mobile phase while the less adsorbed component remains almost stationary.
संबंधित प्रश्न
The best and latest technique for isolation, purification and separation of organic compounds is ______.
Name the different types of chromatographic techniques.
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
Name the different types of Chromatography and explain the principles underlying them.
Compare TLC and Paper Chromatography techniques.
Chromatography is based on the principle of ______.
During hearing of a court case, the judge suspected that some changes in the documents had been carried out. He asked the forensic department to check the ink used at two different places. According to you which technique can give the best results?
The principle involved in paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
In chromatography, the mobile phase is usually ______.
