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प्रश्न
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
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उत्तर
- Thin-layer chromatography (TLC): If components are colourless but have the property of fluorescence then they can be visualized under UV light, or the plate can be kept in a chamber containing a few iodine crystals. The iodine vapours are adsorbed by the components and the spots appear brown. Also, a spraying agent like ninhydrin can also be used (for amino acids).
- Paper Chromatography: The spots of the separated colourless components may be observed either under ultra-violet light or by the use of an appropriate spraying agent.
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संबंधित प्रश्न
Give a brief description of the principle of the following technique taking an example.
Chromatography
The best and latest technique for isolation, purification and separation of organic compounds is ______.
What is chromatography?
What are the advantages of chromatography?
Name the simplest type of chromatography?
What is meant by stationary phase and mobile phase in chromatography?
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Compare TLC and Paper Chromatography techniques.
Chromatography is based on the principle of ______.
Paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
In chromatography, which of the following statement is INCORRECT for Rf?
Who first described the method of chromatography?
