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प्रश्न
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
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उत्तर
- Thin-layer chromatography (TLC): If components are colourless but have the property of fluorescence then they can be visualized under UV light, or the plate can be kept in a chamber containing a few iodine crystals. The iodine vapours are adsorbed by the components and the spots appear brown. Also, a spraying agent like ninhydrin can also be used (for amino acids).
- Paper Chromatography: The spots of the separated colourless components may be observed either under ultra-violet light or by the use of an appropriate spraying agent.
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संबंधित प्रश्न
Give a brief description of the principle of the following technique taking an example.
Chromatography
What is chromatography?
What are the advantages of chromatography?
Name the simplest type of chromatography?
What is meant by stationary phase and mobile phase in chromatography?
Select the correct alternative from the choices given for the following statement:
In chromatography the filter paper is
Give a scientific reason.
In chromatography, the ingredients of a mixture rise up to limited height water rises up to the upper end of the paper.
State the correct technique for the separation of the following mixture.
solid constituents in a liquid constituent by adsorption.
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
Name the different types of Chromatography and explain the principles underlying them.
Compare TLC and Paper Chromatography techniques.
Label the diagram and explain the process in your words.
The principle involved in paper chromatography is ______.
Paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
In chromatography, which of the following statement is INCORRECT for Rf?
In chromatography, the mobile phase is usually ______.
Who first described the method of chromatography?
