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प्रश्न
How do you visualize colourless compounds after separation in TLC and Paper Chromatography?
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उत्तर
- Thin-layer chromatography (TLC): If components are colourless but have the property of fluorescence then they can be visualized under UV light, or the plate can be kept in a chamber containing a few iodine crystals. The iodine vapours are adsorbed by the components and the spots appear brown. Also, a spraying agent like ninhydrin can also be used (for amino acids).
- Paper Chromatography: The spots of the separated colourless components may be observed either under ultra-violet light or by the use of an appropriate spraying agent.
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संबंधित प्रश्न
Explain the principle of paper chromatography.
What is chromatography?
Name the simplest type of chromatography?
What is meant by stationary phase and mobile phase in chromatography?
Select the correct alternative from the choices given for the following statement:
In chromatography the filter paper is
Dyes of an ink
- Give the principle involved in the separation of the mixture
- Give the technique of separation of the mixture.
Give a reason for the following statement:
In chromatography the absorbent medium e.g. What man filter paper is known as the stationary phase.
Choose the correct option.
Colourless components on chromatogram can not be observed by the following:
Give names of two materials used as stationary phase in chromatography.
Define the Stationary phase.
Name the different types of Chromatography and explain the principles underlying them.
Compare TLC and Paper Chromatography techniques.
Label the diagram and explain the process in your words.
Paper chromatography is ______.
In chromatography technique, the purification of compound is independent of ______.
The separation of two coloured substances was done by paper chromatography. The distances travelled by solvent front, substance A and substance B from the baseline are 3.25 cm, 2.08 cm and 1.05 cm respectively. The ratio of Rf values of A to B is ______.
In chromatography, which of the following statement is INCORRECT for Rf?
Which basic principle is used in chromatography?
In chromatography, the mobile phase is usually ______.
Who first described the method of chromatography?
