HSC Science (General) 12th Standard Board Exam - Maharashtra State Board Important Questions for Biology

cDNA means ______.

Give the applications of the PCR technique.

The recognition sequence of restriction enzymes is generally ______ nucleotide long.

Explain the properties of a good or ideal cloning vector for rDNA technology.

In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.

Ti plasmid being used for introducing genes in plants obtained from ______.

What is plasmid?

What is Biopiracy? 

Define biotechnology? Which are the basic principles and process of biotechnology?

What is gene cloning? Explain different tools used for it.

What is Recognition sequence? Explain in brief.

What is PCR? Explain different steps involved in it.

Explain the following terms with respect to rDNA technology

1. passanger DNA
2. Chimeric DNA
3. Transformed cell
4. restriction site

Annealing step of PCR operates at ______ °C.

Name the plant disease caused by Agrobacterium tumefaciens.

What are the effects of biotechnology with relation to human health?

Explain any three examples of biopiracy.

Which of the following is the correct recognition sequence of restriction enzyme Eco RI?

Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:

5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'

1. Name a restriction enzyme which can recognise this DNA sequence.
2. Write the sequence after digestion.
3. Why are the ends generated after digestion called sticky ends?

Which vector is mostly used in rDNA technology in plants?