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Question
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
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Solution
- Passenger DNA: A DNA fragment containing desired gene that is isolated and selected for cloning is called foreign DNA or passenger DNA.
- Chimeric DNA: The combination of vector DNA and foreign DNA is called Recombinant DNA or Chimeric DNA.
- Transformed cell: The host/ competent cell which has taken up rDNA is called transformed cell.
- Restriction site: The particular sequence of nucleotides on DNA where a restriction enzyme cuts the two strands of DNA is called recognition or restriction site.
RELATED QUESTIONS
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Explain the steps in process of rDNA technology with suitable diagrams.
Which of the following term represents pairing of primers to the ssDNA segment during PCR?
Study the following statements regarding cDNA library and select the correct option.
- Study the following statements regarding cDNA library and select the correct option.
- DNA is produced from isolated rRNA by reverse transcription. The DNA so made is called complementary DNA (cDNA).
Which of the following is used for the isolation and purification of the desired product produced by transformed cells?
A ______ refers to the competent host cell that has taken up chimeric DNA.
During gene cloning, the enzyme ______ is used to join the gene of interest with the plasmid vector.
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Name the following:
Human protein produced by rDNA technology to treat cancer.
Which one of the following is NOT true regarding restriction endonucleases?
They are ______.
