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प्रश्न
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
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उत्तर
- Passenger DNA: A DNA fragment containing desired gene that is isolated and selected for cloning is called foreign DNA or passenger DNA.
- Chimeric DNA: The combination of vector DNA and foreign DNA is called Recombinant DNA or Chimeric DNA.
- Transformed cell: The host/ competent cell which has taken up rDNA is called transformed cell.
- Restriction site: The particular sequence of nucleotides on DNA where a restriction enzyme cuts the two strands of DNA is called recognition or restriction site.
संबंधित प्रश्न
Recombinant protein .................. is used to dissolve blood clots present in the body.
In the process of rDNA technology, if two separate restriction enzymes are used to cut vector and donor DNA then which problem will arise in the formation of rDNA or chimeric DNA? Explain.
Study the following statements regarding cDNA library and select the correct option.
- Study the following statements regarding cDNA library and select the correct option.
- DNA is produced from isolated rRNA by reverse transcription. The DNA so made is called complementary DNA (cDNA).
Which of the following technique is used for the separation of DNA fragments of different lengths?
Identify the CORRECT statements regarding edible vaccines.
Lambda phage which is commonly used as a cloning vector in rDNA technology usually infects ____________.
Identify the process in Column I with the term in Column II and select the correct option.
| Column I | Column II |
| A. Amplification of gene of interest | I. Downstream processing |
| B. Large-scale production of plants in a short duration. | II. Polymerase chain reaction |
| C. Separation and purification of the desired product | III. Transformation |
| D. Insertion of rDNA into the host | IV. Micropropagation |
Give reason: All the fragments of a genome are cloned for storing them in genomic library.
Name the following:
Human protein produced by rDNA technology to treat cancer.
Plasmid DNA containing foreign DNA is called______.
