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प्रश्न
Explain the following terms with respect to rDNA technology
- passanger DNA
- Chimeric DNA
- Transformed cell
- restriction site
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उत्तर
- Passenger DNA: A DNA fragment containing desired gene that is isolated and selected for cloning is called foreign DNA or passenger DNA.
- Chimeric DNA: The combination of vector DNA and foreign DNA is called Recombinant DNA or Chimeric DNA.
- Transformed cell: The host/ competent cell which has taken up rDNA is called transformed cell.
- Restriction site: The particular sequence of nucleotides on DNA where a restriction enzyme cuts the two strands of DNA is called recognition or restriction site.
संबंधित प्रश्न
Explain the steps in process of rDNA technology with suitable diagrams.
Study the following statements regarding proteins that are being produced by transgenic crop plants and select the correct option.
i. Human testosterone hormone is produced by gene inserted into the chloroplast DNA of tobacco plants.
ii. Protein antigens to be used in vaccines for e.g. Patient-specific antilymphoma (a cancer) vaccines.
iii. In transgenic plants, Humanized antibodies can be produced against HIV, Respiratory syncytial virus (RSV), Herpes simplex virus (HSY).
Which of the following is used for assisting the transfer of rDNA into a bacterial cell?
Identify the correct term used to represent the DNA molecule formed by joining a gene coding for insulin to a plasmid pBR322 by using DNA ligase.
Which of the following is used for the isolation and purification of the desired product produced by transformed cells?
Which of the following is/are the CORRECT step/s involved in construction of a recombinant DNA?
During gene cloning, the enzyme ______ is used to join the gene of interest with the plasmid vector.
Identify the process by which competent host can take up chimeric DNA.
Give reason: All the fragments of a genome are cloned for storing them in genomic library.
Plasmid DNA containing foreign DNA is called______.
