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Why are the biocontrol agents preferred over the chemical pesticides?
Concept: Microbes as Biocontrol Agents
Why are some molecules called bioactive molecules?
Concept: Microbes in Industrial Products
Mention the respective source and state the specific use of any two bioactive molecules.
Concept: Microbes in Industrial Products
What does PCR stand for? Describe the different steps of PCR
Concept: Principles of Biotechnology
Give an account of the Blue-White Method of selection of recombinants.
Concept: Principles of Biotechnology
Write a short note on Electrophoresis.
Concept: Principles of Biotechnology
Expand the following abbreviation: BAC
Concept: Principles of Biotechnology
Give one significant difference between:
Electroporation and Gene Gun.
Concept: Principles of Biotechnology
What are the restriction endonucleases? Give the rules of their nomenclature.
Concept: Principles of Biotechnology
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Concept: Principles of Biotechnology
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Concept: Principles of Biotechnology
Describe two vectors less methods of gene transfer used in rDNA technology.
Concept: Principles of Biotechnology
Why are bio-fertilizers preferred over chemical fertilizers?
Concept: Principles of Biotechnology
What is the role of a probe in recombinant DNA technology?
Concept: Genetic Engineering
Name and describe the technique that helps in separation of DNA fragments.
Concept: Principles of Biotechnology
Which molecules are called molecular scissors? Why are they called so?
Concept: Principles of Biotechnology
The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
Concept: Principles of Biotechnology
Name the source of thermostable DNA polymerase.
Concept: Principles of Biotechnology
How many sets of primers are required in each cycle of PCR?
Concept: Principles of Biotechnology
Which one of the following enzymes is used to join DNA fragments?
Concept: Principles of Biotechnology
