ISC (Science) ISC Class 12 - CISCE Important Questions

List any four applications of tissue culture.

Give one significant difference between:

Electroporation and Gene Gun.

Give one significant difference between :

ECG and EEG

What are the restriction endonucleases? Give the rules of their nomenclature.

Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.

Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.

Describe two vectors less methods of gene transfer used in rDNA technology.

Why are bio-fertilizers preferred over chemical fertilizers?

What is the role of a probe in recombinant DNA technology?

Name and describe the technique that helps in separation of DNA fragments.

Which molecules are called molecular scissors? Why are they called so?

Explain the steps involved in the process of plant breeding.

The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.

Name the source of thermostable DNA polymerase.

How many sets of primers are required in each cycle of PCR?

Which one of the following enzymes is used to join DNA fragments? 

Give a reason for the following:

DNA cannot enter directly into the host cell.

State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.

Study the diagram given below and answer the questions that follow.

1. Name the cloning vector shown above. In which organism in this cloning vector inserted?

2. Mention any two restriction sites shown in the diagram.

3. Name any two selectable markers shown in the diagram.

Give the name of the target pest of gene cry 1 Ac.