Advertisements
Advertisements
Question
During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine. What would be the consequence?
Advertisements
Solution
Further polymerisation would not occur, as the 3’ OH on sugar is not there to add a new nucleotide for forming ester bond.
APPEARS IN
RELATED QUESTIONS
Group the following as nitrogenous bases and nucleosides:
Adenine, Cytidine, Thymine, Guanosine, Uracil and Cytosine.
Which property of DNA double helix led Watson and Crick to hypothesise semi-conservative mode of DNA replication? Explain.
Chargaff’s rules are applicable to ______.
If a double stranded DNA has 20% of cytosine, what will be the percentage of adenine in it?
In some viruses, RNA is present instead of DNA indicating that ______.
In a DNA strand the nucleotides are linked together by ______.
Who amongst the following scientists had no contribution in the development of the double helix model for the structure of DNA?
What is the nature of the strands of the DNA duplex?
How far is each base pair from the next one in DNA double helix model?
If 30 percent of an organism’s DNA is thymine, then ______.
H-bonds between Cytosine and Guanine are ______.

DNA is made of two chains that twist about one another in the shape of a ______.
______ occurs in RNA instead of the nitrogenous base thymine found in DNA.
Which ratio is constant for DNA?
Which of the following is a structural subunit of DNA?
Which of the following is a purine?
Retroviruses do not follow central Dogma. Comment.
A short piece of DNA, having 20 base pairs, was analyzed to find the number of nucleotide bases in each of the polynucleotide strands. Some of the results are shown in the table.
| Number of nucleotide bases | ||||
| Adenine | Cytosine | Guanine | Thymine | |
| Strand 1 | 4 | 4 | ||
| Strand 2 | 5 | |||
How many nucleotides containing Adenine were present in strand 2?
