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प्रश्न
During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine. What would be the consequence?
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उत्तर
Further polymerisation would not occur, as the 3’ OH on sugar is not there to add a new nucleotide for forming ester bond.
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संबंधित प्रश्न
Group the following as nitrogenous bases and nucleosides:
Adenine, Cytidine, Thymine, Guanosine, Uracil and Cytosine.
Which property of DNA double helix led Watson and Crick to hypothesise semi-conservative mode of DNA replication? Explain.
Chargaff’s rules are applicable to ______.
In a DNA strand the nucleotides are linked together by ______.
While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering the Chargaff’s rule it can be concluded that ______.
How far is each base pair from the next one in DNA double helix model?
Synthesis of DNA from RNA is explained by ______.
If 30 percent of an organism’s DNA is thymine, then ______.
A peptide chain assumes secondary structure through the formation of ______.
Which of the following is a purine?
A short piece of DNA, having 20 base pairs, was analyzed to find the number of nucleotide bases in each of the polynucleotide strands. Some of the results are shown in the table.
| Number of nucleotide bases | ||||
| Adenine | Cytosine | Guanine | Thymine | |
| Strand 1 | 4 | 4 | ||
| Strand 2 | 5 | |||
How many nucleotides containing Adenine were present in strand 2?
Polymerase chain reaction (PCR) amplifies DNA following the equation.
What structural difference exists between the pentose sugar found in DNA and the pentose sugar found in RNA?
In a polynucleotide chain, nucleotides are linked together by which type of bond?
The backbone of a polynucleotide chain is formed by which components?
A nucleotide is the monomer unit of a nucleic acid. Which of the following correctly identifies the three components of a nucleotide?
