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प्रश्न
If a double stranded DNA has 20 per cent of cytosine, calculate the per cent of adenine in the DNA.
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उत्तर
According to Chargaff’s rule, the DNA molecule should have an equal ratio of pyrimidine (cytosine and thymine) and purine (adenine and guanine). It means that the number of adenine molecules is equal to thymine molecules and the number of guanine molecules is equal to cytosine molecules.
% A = % T and % G = % C
If double stranded DNA has 20% of cytosine, then according to the law, it would have 20% of guanine.
Thus, percentage of G + C content = 40%
The remaining 60% represents both A + T molecules. Since adenine and guanine are always present in equal numbers, the percentage of adenine molecule is 30%.
संबंधित प्रश्न
Which property of DNA double helix led Watson and Crick to hypothesise semi-conservative mode of DNA replication? Explain.
Chargaff’s rules are applicable to ______.
One turn of DNA possesses ______.
Both deoxyribose and ribose belong to a class of sugars called ______.
The fact that a purine base always paired through hydrogen bonds with a pyrimidine base leads to, in the DNA double helix ______.
Who amongst the following scientists had no contribution in the development of the double helix model for the structure of DNA?
While analysing the DNA of an organism a total number of 5386 nucleotides were found out of which the proportion of different bases were: Adenine = 29%, Guanine = 17%, Cytosine = 32%, Thymine = 17%. Considering the Chargaff’s rule it can be concluded that ______.
What is the nature of the strands of the DNA duplex?
Protein helping in opening of DNA double helix in front of replications fork is ______.
How far is each base pair from the next one in DNA double helix model?
Synthesis of DNA from RNA is explained by ______.
If 30 percent of an organism’s DNA is thymine, then ______.
H-bonds between Cytosine and Guanine are ______.
By which bonds the purine and pyrimidine pairs of Complementary Strands of DNA held together?
There is no DNA in ______.
DNA is made of two chains that twist about one another in the shape of a ______.
______ occurs in RNA instead of the nitrogenous base thymine found in DNA.
A peptide chain assumes secondary structure through the formation of ______.
In the polynucleotide chain of DNA, a nitrogenous base is linked to the -OH of ______
Which of the following is a purine?
Retroviruses do not follow central Dogma. Comment.
During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine. What would be the consequence?
A short piece of DNA, having 20 base pairs, was analyzed to find the number of nucleotide bases in each of the polynucleotide strands. Some of the results are shown in the table.
| Number of nucleotide bases | ||||
| Adenine | Cytosine | Guanine | Thymine | |
| Strand 1 | 4 | 4 | ||
| Strand 2 | 5 | |||
How many nucleotides containing Adenine were present in strand 2?
Polymerase chain reaction (PCR) amplifies DNA following the equation.
