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प्रश्न
During in vitro synthesis of DNA, a researcher used 2’, 3’ – dideoxy cytidine triphosphate as raw nucleotide in place of 2’-deoxy cytidine. What would be the consequence?
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उत्तर
Further polymerisation would not occur, as the 3’ OH on sugar is not there to add a new nucleotide for forming ester bond.
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संबंधित प्रश्न
Group the following as nitrogenous bases and nucleosides:
Adenine, Cytidine, Thymine, Guanosine, Uracil and Cytosine.
If a double stranded DNA has 20 per cent of cytosine, calculate the per cent of adenine in the DNA.
Chargaff’s rules are applicable to ______.
In some viruses, RNA is present instead of DNA indicating that ______.
In a DNA strand the nucleotides are linked together by ______.
A nucleoside differs from a nucleotide. It lacks the ______.
Both deoxyribose and ribose belong to a class of sugars called ______.
The fact that a purine base always paired through hydrogen bonds with a pyrimidine base leads to, in the DNA double helix ______.
Protein helping in opening of DNA double helix in front of replications fork is ______.
Synthesis of DNA from RNA is explained by ______.
H-bonds between Cytosine and Guanine are ______.
DNA is made of two chains that twist about one another in the shape of a ______.
______ occurs in RNA instead of the nitrogenous base thymine found in DNA.
A peptide chain assumes secondary structure through the formation of ______.
In the polynucleotide chain of DNA, a nitrogenous base is linked to the -OH of ______
Which of the following is a purine?
Retroviruses do not follow central Dogma. Comment.
What is the smallest part of a DNA molecule that can be changed by a point mutation?
