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Question
Describe the process of transcription in bacteria
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Solution
Transcription has three steps—initiation, elongation, and termination.
Initiation:
RNA polymerase binds with the promoter to initiate the process of transcription. Association with the initiation factor (σ) alters the specificity of RNA polymerase to initiate transcription.
Elongation:
RNA polymerase uses nucleoside triphosphate as the substrate, and polymerisation occurs according to complementarity.
Termination:
Termination occurs when the termination factor (rho) alters the specificity of RNA polymerase to terminate transcription. As the RNA polymerase proceeds to perform elongation, a short stretch of RNA remains bound to the enzyme. As the enzyme reaches the termination region, this nascent RNA falls off and transcription is terminated.
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