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Question
Meselson and Stahl’s experiment proved ______.
Options
Transduction
Transformation
DNA is the genetic material.
Semi-conservative nature of DNA replication.
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Solution
Meselson and Stahl’s experiment proved Semi-conservative nature of DNA replication.
Explanation:
The Meselson and Stahl experiment (1958) definitively proved that DNA replication is semiconservative, meaning each new DNA molecule consists of one original parental strand and one newly synthesised strand. By growing E. coli bacteria in heavy nitrogen (15N) and then switching them to a medium with light nitrogen (14N), the researchers used density gradient centrifugation to observe the DNA’s weight over generations. They found that after one generation, all DNA was of intermediate density (a hybrid of heavy and light), and after two generations, half the DNA was hybrid and half was entirely light, which confirmed that the parent strands separate and serve as templates for new matching strands.
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Long Answer Question:
Explain the process of DNA replication.
During DNA replication, the separated strands of DNA are prevented from recoiling by
Draw neat and labelled diagram of Replication Fork.
How Meselson and Stahl explained the concept of Semiconservative Replication of DNA experimentally?
E. coli cell grown on 15N medium are transferred to 14N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?
Identify the direction of DNA replication in eukaryotes.
DNA replication begins at specific sites situated on the molecule. Such sites are called ____________.
Meselson and Stahl used ____________ in the experiment to prove semiconservative DNA replication.
For which of the following purpose agarose gel electrophoresis technique is used?
Enzyme ______ brings about the DNA unwinding by breaking weak hydrogen bonds in the vicinity of origin.
Read the following statements and select the correct option.
Statement I: DNA replication is continuous on leading strand while on the lagging strand it is discontinuous.
Statement II: DNA polymerase cannot initialize polymerization without a primer.
Select the mis-matched pair.
Draw a suitable diagram of replication of eukaryotic DNA and label any three parts.
Which of the following DNA polymerase enzyme in eukaryotes has 5' -3' exonuclease activity?
Which of the following statement is correct regarding the process of replication in E.coli?
In eukaryotic organisms, replication of DNA occurs in ______.
Statement I - In prokaryotes, there is only replicon however in eukaryotes, these are several replicons in tandem.
Statement II - Two separated strands in a replicon of DNA are prevented from re-joining by helicase enzyme.
In light of above statements, choose the correct answer from the option given below.
