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प्रश्न
Meselson and Stahl’s experiment proved ______.
विकल्प
Transduction
Transformation
DNA is the genetic material.
Semi-conservative nature of DNA replication.
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उत्तर
Meselson and Stahl’s experiment proved Semi-conservative nature of DNA replication.
Explanation:
The Meselson and Stahl experiment (1958) definitively proved that DNA replication is semiconservative, meaning each new DNA molecule consists of one original parental strand and one newly synthesised strand. By growing E. coli bacteria in heavy nitrogen (15N) and then switching them to a medium with light nitrogen (14N), the researchers used density gradient centrifugation to observe the DNA’s weight over generations. They found that after one generation, all DNA was of intermediate density (a hybrid of heavy and light), and after two generations, half the DNA was hybrid and half was entirely light, which confirmed that the parent strands separate and serve as templates for new matching strands.
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संबंधित प्रश्न
Long Answer Question:
Explain the process of DNA replication.
Define Heterochromatin.
Describe the process of semiconservative replication of DNA with the help of a neat and labelled diagram.
E. coli cell grown on 15N medium are transferred to 14N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?
What is the basis for the difference in the synthesis of the leading and lagging strand of DNA molecules?
DNA replication begins at specific sites situated on the molecule. Such sites are called ____________.
Enzyme ______ brings about the DNA unwinding by breaking weak hydrogen bonds in the vicinity of origin.
Identify the CORRECT combination of statements with respect to DNA synthesis.
I. Always the direction of DNA polymerization 5' → 3' when referring to the polarity of strand being synthesized.
II. DNA ligase forms hydrogen bonds between two newly synthesized DNA strands.
III. DNA polymerases on their own cannot initiate the process of replication.
IV. DNA polymerase can catalyse polymerization in both 5'→ 3' and 3'→ 5' direction.
Which of the following is present at the sticky ends of a fragmented DNA molecule?
Which of the following is the function of single-strand binding proteins?
Select the mis-matched pair.
"DNA is considered as genetic material" - Why?
Wobble position means:
Identify the biomolecule that is capable of self-replication.
What background information did Watson and Crick had available with them for developing a model of DNA? What was their own contribution?
Characteristics unique to DNA are ______.
Which of the following DNA polymerase enzyme in eukaryotes has 5' -3' exonuclease activity?
Enzyme involved in synthesis of RNA primer.
Statement I - In prokaryotes, there is only replicon however in eukaryotes, these are several replicons in tandem.
Statement II - Two separated strands in a replicon of DNA are prevented from re-joining by helicase enzyme.
In light of above statements, choose the correct answer from the option given below.
