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Which molecules are called molecular scissors? Why are they called so?
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The rDNA technology has provided a method to control the nematode parasite Meloidogyne incognita. Explain the principle involved in this technique.
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Name the source of thermostable DNA polymerase.
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How many sets of primers are required in each cycle of PCR?
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Which one of the following enzymes is used to join DNA fragments?
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Give a reason for the following:
DNA cannot enter directly into the host cell.
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State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
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Study the diagram given below and answer the questions that follow.

- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
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Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
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Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
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David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
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Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.

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Define Racemose inflorescence
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Name a plant which flowers every twelve years.
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Name the terminal stage of ageing in the life cycle of plants.
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Give a reason for the Following:
Pollen grains of wind-pollinated flowers are produced in large quantities.
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Define the Stenothermal organisms.
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Give the biological name of the following:
The mould from which penicillin is obtained.
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Explain the different types of endosperms in angiosperms.
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