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Revision: Plant Physiology >> Photosynthesis Biology (English Medium) ICSE Class 10 CISCE

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Definitions [9]

Define the following term:

Photolysis of water

Releasing electrons and dividing the water molecule (H2O) into its two components (Hydrogen and Oxygen). Photolysis is the term used to describe this reaction, which is characterised by the fracturing of molecules by light (photo = light, lysis = breaking).

Definition: Photosynthesis

Photosynthesis is the process by which living plant cells, containing chlorophyll, produce food substances (glucose and starch) from carbon dioxide and water by using light energy. Plants release oxygen as a byproduct during photosynthesis.

Define the following term:

Chloroplast

Chloroplasts are indeed miniature ovoid structures that are encased in a double membrane. Nevertheless, the response could offer a more succinct definition: Chloroplasts are the cellular organelles that are responsible for the process of photosynthesis in plant cells. They are the sites where photosynthesis occurs and contain thylakoids.

Define the following term:

Thylakoids

Thylakoids are indeed very small compartments that are present within chloroplasts. They are the locations where the light-dependent reactions of photosynthesis take place and contain chlorophyll. Nevertheless, the response could be simplified to enhance its clarity.

Define Photosynthesis.

In the presence of chlorophyll, photosynthesis is the process by which green plants and certain other organisms convert carbon dioxide and water into glucose (a form of sugar) using light energy, typically from the sun. As a byproduct, this process generates oxygen.

Definition: Photophosphorylation

Photophosphorylation is the process of converting ADP into energy-rich ATP by adding an inorganic phosphate (Pi), using energy from light (photons).

Definition: Photolysis

Photolysis occurs in the grana of a chloroplast and is defined as the splitting of H2O molecules into hydrogen ions and oxygen in the presence of light.

Definition: Polymerisation

The process of conversion of many simpler and smaller molecules into a complex, bigger molecule is termed as polymerisation. For example, conversion of several glucose molecules into a starch molecule. 

Define the following term:

Polymerisation

Most green plants use glucose as soon as it occurs during photosynthesis to make starch. Polymerisation is the process by which several glucose molecules are turned into one starch molecule.

The process by which monomer molecules combine together to form a polymer is called polymerisation.

The process by which monomer molecules combine together to form a polymer is called polymerisation.

Chemica Equations [1]

Chemical Equation: Photosynthesis

The balanced chemical equation is: \[6\mathrm{CO}_{2}+12\mathrm{H}_{2}\mathrm{O}\frac{\text{light energy}}{\text{chlorophyll}}\mathrm{C}_{6}\mathrm{H}_{12}\mathrm{O}_{6}+6\mathrm{H}_{2}\mathrm{O}+6\mathrm{O}_{2}\uparrow\]

Key Points

Key Points: Concept of Photosynthesis
  • Photosynthesis converts sunlight into chemical energy using CO₂ and water, producing glucose and releasing oxygen as a byproduct.
  • Green plants are autotrophs (make their own food); all other organisms are heterotrophs. All life depends on sunlight for energy.
  • Three essentials for photosynthesis: chlorophyll, light, and CO₂.
  • Experiment 1 - Variegated leaf tested for starch showed that photosynthesis occurs only in green parts in the presence of light.
  • Experiment 2 - Leaf part enclosed with KOH-soaked cotton (absorbs CO₂) tested negative for starch → proved CO₂ is necessary for photosynthesis.
Key Points: Chlorophyll
  1. Chlorophyll is the green pigment in plants, found in chloroplasts.
  2. It is located in the thylakoid walls inside the chloroplasts.
  3. Chloroplasts are mainly present in mesophyll cells of leaves.
  4. There are nine types of chlorophyll, but chlorophyll a and chlorophyll b are the most common.
  5. Chlorophyll absorbs blue and red light for photosynthesis and reflects green light.
Key Points: Stomatal Function and Movement
  1. Stomata are tiny pores mostly on the underside of leaves, responsible for CO₂ intake for photosynthesis.
  2. In darkness, stomata close to reduce water loss by transpiration; in light, they reopen to allow CO₂ entry.
  3. Transpiration occurs alongside photosynthesis, thus "transpiration is the price the plant pays for photosynthesis."
  4. The opening and closing of stomata depend on the movement of water in and out of guard cells, which have a thick inner and thin outer wall.
Key Points: Opening and Closing of Stomata (Theories)
  1. Two main theories explain stomatal movement: the potassium ion concentration theory (recent) and the Sugar concentration theory (old).
  2. K⁺ Ion Theory: In light, ATP from photosynthesis pumps K⁺ ions into guard cells, increasing osmotic pressure, causing water inflow and stomatal opening.
  3. At night, K⁺ ions leak out, guard cells lose turgor, become flaccid, and the stomata close.
  4. Sugar Theory: Photosynthesis produces glucose, which increases osmotic pressure in guard cells, leading to water uptake and turgidity, opening the stomata.
  5. In both theories, stomatal closure occurs when water is lost from guard cells, making them flaccid and closing the pore.
Key Points: Process of Photosynthesis
  1. Photosynthesis mainly occurs in mesophyll cells (palisade and spongy) of leaves, using chlorophyll to trap sunlight.
  2. Carbon dioxide enters the leaf via stomata by diffusion down a concentration gradient.
  3. Water is absorbed by roots, transported via the stem to mesophyll cells of the leaves.
  4. Using light energy, chlorophyll helps synthesize glucose (C₆H₁₂O₆) from CO₂ and H₂O, releasing O₂ as a by-product.
  5. The balanced chemical equation is: \[6\mathrm{CO}_{2}+12\mathrm{H}_{2}\mathrm{O}\frac{\text{light energy}}{\text{chlorophyll}}\mathrm{C}_{6}\mathrm{H}_{12}\mathrm{O}_{6}+6\mathrm{H}_{2}\mathrm{O}+6\mathrm{O}_{2}\uparrow\]
Key Points: Light Reaction
  • Light Harvesting Complexes (LHC) - Made up of hundreds of pigment molecules bound to proteins. Found in PS I and PS II. Help absorb different wavelengths of light for efficient photosynthesis.
  • Antennae System - In each photosystem, all pigments except one chlorophyll a molecule form the antennae (light-harvesting system). They absorb light and pass energy to the reaction centre.
  • Reaction Centre - The single chlorophyll a molecule that directly participates in the photochemical reaction. It is different in PS I and PS II.
  • PS I - Reaction centre = P700 (absorbs light at 700 nm).
  • PS II - Reaction centre = P680 (absorbs light at 680 nm).
  • Steps of Light Reaction - Light absorption → Water splitting → Oxygen release → ATP and NADPH production.
Key Points: Products of Photosynthesis
  1. Glucose is used immediately, stored as starch, converted into sucrose, or used to make fats and proteins.
  2. Water produced may be reused in further photosynthesis.
  3. Oxygen mostly diffuses out, but some is used in photorespiration.
  4. Synthesized food is temporarily stored as starch in the leaf.
  5. At night, starch is converted back to sugar and translocated to other plant parts.
Key Points: Factors Affecting Photosynthesis
  • Blackman’s Law of Limiting Factors states that the rate of photosynthesis is controlled by the factor in the least supply.
  • Light affects photosynthesis through intensity, quality, and duration; it shows a linear increase at low intensity and saturation at about 10% of full sunlight.
  • Carbon dioxide is the major limiting factor; increasing CO₂ concentration increases photosynthesis up to a limit, after which it may become harmful.
  • Temperature controls enzymatic reactions (dark reactions); C₄ plants work better at higher temperatures, while C₃ plants have a lower optimum temperature.
  • Water affects photosynthesis indirectly; water stress causes stomatal closure, reducing CO₂ availability and decreasing photosynthesis.
  • All factors work together, but usually one limiting factor determines the overall rate of photosynthesis.
Key Points: Experiments on Photosynthesis
  • Priestley (1770) - Plants take up CO₂ and release O₂; plants restore what animals and candles remove from the air.
  • Ingenhousz (1779) - O₂ release occurs only in sunlight and only by the green parts of plants.
  • Theodore de Saussure (1804) - Water is an essential requirement for photosynthesis.
  • Julius von Sachs (1854) - Green parts produce glucose, stored as starch; chlorophyll is located in chloroplasts.
  • T.W. Engelmann (1888) - Plotted the first action spectrum of photosynthesis; bacteria accumulated in blue and red light regions.
  • C.B. van Niel (1931) - Photosynthesis is a light-dependent reaction; H from an oxidisable compound reduces CO₂ to form sugar. O₂ comes from H₂O, not CO₂.
  • Hill (1937), Calvin (1954-55), Hatch & Slack (1965) - Hill: O₂ evolves in light reaction, Calvin: traced carbon fixation pathway, Hatch & Slack: discovered C4 pathway.
Key Points: Carbon Cycle
  • Carbon is a key element of living organisms and is found in air, water, soil, rocks, and oceans (major reservoirs).
  • The carbon cycle is the movement of carbon between the atmosphere, organisms, and the Earth through natural processes.
  • Photosynthesis fixes CO₂ into organic matter, which passes through food chains.
  • CO₂ returns to the atmosphere through respiration, decomposition, and combustion.
  • Carbon sinks & humans: Fossil fuels and rocks store carbon, but human activities increase CO₂, causing global warming.

Important Questions [48]

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