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Question
Using the destarched plant describe step by step how would you proceed to prove that in the absence of light the leaf cannot manufacture starch?
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Solution
- In a dark room, a leaf on the destarched plant is covered on either side with strips of black paper which are kept in place by paper clips or cellophane tape.
- Now the potted plant is kept in the light.
- After a few hours, the leaf is detached from the plant and the black strips are removed.
- Then the leaf is boiled in water for 2-3 minutes and then boiled in alcohol to remove the green chlorophyll.
- The leaf is then washed in water and tested with iodine.
- The region of the leaf that was under the black strip turns yellowish-brown showing that no starch was made, while the rest of the leaf turns bluish-black showing the starch was made in the presence of light.
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