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Question
Enumerate the steps involved in testing a green leaf for the presence of starch.
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Solution
Test to determine the presence of starch in a leaf:
- Dip a leaf in boiling water for a minute to kill the cells.
- Boil the leaf in methylated spirit in a water bath to remove the chlorophyll, till the leaf turns pale blue and becomes hard and brittle.
- Now place the leaf in hot water to soften it.
- Place the leaf in a Petri dish and pour iodine solution over it.
- The appearance of a blue-black colour on the leaf is indicative of the presence of starch.
- The absence of starch is indicated by a brown colouration.
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