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Question
Two healthy green plants were placed in the dark for 24 hours. They were then set up, as shown in the figure, for 4 hours. Then a leaf was taken from each plant and
the chlorophyll was removed from the leaves:
( i) What is the next and final step in this experiment?
( ii ) What would be the results of the final step?
( iii ) Why was it necessary to grease the glass sheet?
( iv ) What hypothesis is being tested in this experiment?

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Solution
(i) The leaf is then treated with iodine solution.
(ii) The leaf from first plant will not show starch test while the leaf from second plant will become blue-black showing the presence of starch.
(iii) It is necessary to grease the glass sheet to prevent the entry of air containing CO2.
(iv) The hypothesis that CO2 is necessary for photosynthesis is being tested in this experiment.
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A potted plant was taken in order to prove a factor necessary for photosynthesis. The potted plant was kept in the dark for 24 hours. One of the leaves was covered with black paper in the centre. The potted plant was then placed in sunlight for a few hours.
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Light reaction and Dark reaction.
Complete the following sentence with appropriate word:
________ are regarded as complete photosynthetic units of plants.
Choose the Correct Answer:
The main difference between chlorophyll ‘a’ and ‘b’ is:
Match the terms given in column A with column B:
| Column A | Column B | ||
| (a) | Chlorophyll | (i) | Removes/absorbs CO2 |
| (b) | Methylated spirit | (ii) | Tests presence of starch |
| (c) | Potassium hydroxide | (iii) | Traps solar energy |
| (d) | Iodine solution | (iv) | Produces oxygen |
| (e) | Water | (v) | Bleaches chlorophyll |
Given below is the figure of an experimental set-up, showing a physiological act of the plants. Study and answer the following questions:

- What is the objective of this experiment?
- Name and define the process shown here.
- Why do we destarch the leaves before performing the experiment?
- How do we destarch the leaves?
- What will be the observation when we pour iodine solution over the bleached experimental leaf?
- Write a well-balanced equation for the above process.
