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Question
Two healthy green plants were placed in the dark for 24 hours. They were then set up, as shown in the figure, for 4 hours. Then a leaf was taken from each plant and
the chlorophyll was removed from the leaves:
( i) What is the next and final step in this experiment?
( ii ) What would be the results of the final step?
( iii ) Why was it necessary to grease the glass sheet?
( iv ) What hypothesis is being tested in this experiment?

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Solution
(i) The leaf is then treated with iodine solution.
(ii) The leaf from first plant will not show starch test while the leaf from second plant will become blue-black showing the presence of starch.
(iii) It is necessary to grease the glass sheet to prevent the entry of air containing CO2.
(iv) The hypothesis that CO2 is necessary for photosynthesis is being tested in this experiment.
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