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Explain the properties of a good or ideal cloning vector for rDNA technology.
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A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
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Give the steps in PCR or polymerase chain reaction with suitable diagrams.
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Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
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Enlist and write in brief about the different biological tools required in rDNA technology.
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Which factor of an ecosystem includes plants, animals, and micro-organisms?
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How is the dormancy of seeds different from hibernation in animals?
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If a marine fish is placed in a freshwater aquarium, will it be able to survive? Give reason.
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Distinguish between the following:
Hibernation and Aestivation
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Distinguish between the following:
Ectotherms and Endotherms
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Observe the graph and select the correct option:

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Short answer type question
Tropical regions exhibit species richness as compared to polar regions. Justify.
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Planaria shows ______ type of nervous system.
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The technique which involves addition or deletion of genes is ______.
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ECoRI is obtained from ______.
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The enzyme restriction endonuclease ______.
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Ti plasmid being used for introducing genes in plants obtained from ______.
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Polymerase chain reaction is most useful in ______.
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What is Palindromic sequence?
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