Advertisements
Advertisements
Question
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Advertisements
Solution
i. If a PCR machine fails to lower the temperature below 70°C then the annealing step (in which primers attach to the respective ends of the DNA template) would be affected.
ii. The annealing step of PCR requires a temperature ranging between 40°C to 60°C therefore if a PCR machine fails to lower the temperature below 70°C, primers will not attach to the DNA templates.
APPEARS IN
RELATED QUESTIONS
Give the applications of the PCR technique.
The bacterium which causes a plant disease called crown gall is _______________.
What is gene cloning? Explain different tools used for it.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
Human insulin is produced by culturing the genetically engineered ______
What is true for plasmid?
Which of the following is not required in the preparation of a recombinant DNA molecule?
Which one of the following enzymes is used to join DNA fragments?
