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Question
a) Identify the figure given below
b) Redraw the structure as a replicating fork and label the parts

c) Write the source of energy for this replication and name the enzyme involved in this process.
d) Mention the differences in the synthesis of protein, based on the polarity of the two template strands.
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Solution
a) Replication fork
b)

c) Deoxy nucleotide, triphosphate acts as a energy source for replication. DNA polymerase is used for replication.
d) mRNA contacting information for protein synthesis will developed from DNA strand having polariy 5 ’ → 3 ’
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______ enzymes are used as biological scissors in r-DNA technology.
Long Answer Question:
Explain the process of DNA replication.
What are Okazaki fragments?
E. coli cell grown on 15N medium are transferred to 14N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?
From their examination of the structure of DNA, What did Watson and Crick infer about the probable mechanism of DNA replication, coding capability, and mutation?
Read the following statements and select the correct option.
i. The unit of DNA in which replication occurs, is called replicon.
ii. In eukaryotes, there is only one replicon however in prokaryotes there are several replicons in tandem.
Identify the CORRECT combination of statements with respect to DNA synthesis.
I. Always the direction of DNA polymerization 5' → 3' when referring to the polarity of strand being synthesized.
II. DNA ligase forms hydrogen bonds between two newly synthesized DNA strands.
III. DNA polymerases on their own cannot initiate the process of replication.
IV. DNA polymerase can catalyse polymerization in both 5'→ 3' and 3'→ 5' direction.
Which of the following statement is incorrect?
Which of the following reaction is required for proofreading during DNA replication by DNA polymerase III?
In prokaryotes, the primers of lagging strands are removed by ______.
