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Question
ATP produced during glycolysis is a result of substrate level phosphorylation. Explain.
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Solution
When the formation of ATP happens by direct transfer of a phosphoryl (PO3) group to ADP from a phosphorylated reactive intermediate, this is called substrate-level phosphorylation. During glycolysis, ATP is formed at two stages because of the direct transfer of the phosphoryl group to ADP.
- When BPGA (bisphosphoglyceric acid) is converted into PGA (phosphoglyceric acid).
- When PEP (phosphoenolpyruvate) is converted into pyruvic acid.
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