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Question
ATP produced during glycolysis is a result of substrate level phosphorylation. Explain.
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Solution
When the formation of ATP happens by direct transfer of a phosphoryl (PO3) group to ADP from a phosphorylated reactive intermediate, this is called substrate-level phosphorylation. During glycolysis, ATP is formed at two stages because of the direct transfer of the phosphoryl group to ADP.
- When BPGA (bisphosphoglyceric acid) is converted into PGA (phosphoglyceric acid).
- When PEP (phosphoenolpyruvate) is converted into pyruvic acid.
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RELATED QUESTIONS
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\[\ce{C6H12O6 ->[Glycolysis] 'P' + 2NADH + H+ -> 2CH3CHOHCOOH + 2NAD+}\]
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\[\ce{C6H12O6 + 2NAD + 2ADP + 2Pi → CH3 - CO - COOH + 2 NADH2 + 2 ATP}\]
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