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Give the biological name of the following:
The fungus that is being developed as a bio-control agent.
Concept: Microbes as Biocontrol Agents
Give the biological name of the following:
A symbiotic nitrogen-fixing bacterium found in root nodules.
Concept: Microbes in Household Products
Which one of the following factors is responsible for activation of protoxin into active Bt toxin of Bacillus thuringiensis?
Concept: Microbes in Household Products
Home-made fruit juices are turbid, while the bottled ,fruit juices purchased from the market are clear. Give a reason for this difference.
Concept: Microbes in Household Products
Read the passage given below carefully and answer the questions that follow:
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The bacterium Bacillus thuringiensis serovar israelensis (Bti) is commercially prepared in various formulations such as liquid, water dispersible granules, powders, and pellets. It is used as a larvicide all over the world due to its ability to produce a toxic protein that primarily targets the larvae of mosquitoes. In Sweden, Bti has been applied on a large scale in the form of commercially available granular formulation Vecto Bac G (Valent BioScience, USA). The applications have taken place in the lower Dalalven River Area to control mass outbreaks of the floodplain mosquito Aedes sticticus. |
- Why does the toxic protein kill the insects but not the bacterium?
- Can the formulation Vecto Bac G be used to eliminate the species of mosquito referred to above? Why?
Concept: Microbes in Household Products
Why are the biocontrol agents preferred over the chemical pesticides?
Concept: Microbes as Biocontrol Agents
Why are some molecules called bioactive molecules?
Concept: Microbes in Industrial Products
Mention the respective source and state the specific use of any two bioactive molecules.
Concept: Microbes in Industrial Products
What does PCR stand for? Describe the different steps of PCR
Concept: Principles of Processes of Biotechnology
Give an account of the Blue-White Method of selection of recombinants.
Concept: Principles of Processes of Biotechnology
Write a short note on Electrophoresis.
Concept: Principles of Processes of Biotechnology
Expand the following abbreviation: BAC
Concept: Principles of Processes of Biotechnology
Give one significant difference between:
Electroporation and Gene Gun.
Concept: Principles of Processes of Biotechnology
What are the restriction endonucleases? Give the rules of their nomenclature.
Concept: Principles of Processes of Biotechnology
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Concept: Principles of Processes of Biotechnology
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
Concept: Principles of Processes of Biotechnology
Describe two vectors less methods of gene transfer used in rDNA technology.
Concept: Principles of Processes of Biotechnology
Why are bio-fertilizers preferred over chemical fertilizers?
Concept: Principles of Processes of Biotechnology
What is the role of a probe in recombinant DNA technology?
Concept: Genetic Engineering
Name and describe the technique that helps in separation of DNA fragments.
Concept: Principles of Processes of Biotechnology
