Question

Which of the following is the correct recognition sequence of restriction enzyme hind III?

Options

• ^5'---A-A-G-C-T-T---^{3'
  3'}---T-T-C-G-A-A---^5'

• ^5'---G-A-A-T-T-C---^{3'
  3'}---C-T-T-A-A-G---^5'

• ^5'---C-G-A-T-T-C---^{3'
  3'}---G-C-T-A-A-G---^5'

• ^5'---G-G-C-C---^{3'
  3'}---C-C-G-G---^5'

Answer 1

^5'---A-A-G-C-T-T---^3'
3'---T-T-C-G-A-A---^5'

Explanation:

It cuts between the two adenine (A) bases, producing sticky ends. HindIII specifically identifies and cuts the palindromic sequence AAGCTT, ensuring precision in DNA manipulation by targeting this unique sequence.