Advertisements
Advertisements
Question
Name the most commonly used bioreactor and describe its working.
Advertisements
Solution
The most commonly used bioreactor is simple stirred type bioreactor. A stirred-tank reactor is usually cylindrical or with a curved base to facilitate the mixing of the reactor contents. The stirrer facilitates even mixing and oxygen availability throughout the bioreactor. Alternatively, air can be bubbled through the reactor. The bioreactor has an agitator system, an oxygen delivery system and a foam control system, a temperature control system, pH control system and sampling ports so that small volumes of the culture can be withdrawn periodically.
APPEARS IN
RELATED QUESTIONS
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
What is the cell that receives a recombinant gene called?
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
A hind of biotechnology involving manipulation of DNA is ______.
Describe three steps involved in mechanism of PCR.
How does EcoRI differ from an exonuclease?
Give a reason for the following:
DNA cannot enter directly into the host cell.
