Advertisements
Advertisements
Question
Name the most commonly used bioreactor and describe its working.
Advertisements
Solution
The most commonly used bioreactor is simple stirred type bioreactor. A stirred-tank reactor is usually cylindrical or with a curved base to facilitate the mixing of the reactor contents. The stirrer facilitates even mixing and oxygen availability throughout the bioreactor. Alternatively, air can be bubbled through the reactor. The bioreactor has an agitator system, an oxygen delivery system and a foam control system, a temperature control system, pH control system and sampling ports so that small volumes of the culture can be withdrawn periodically.
APPEARS IN
RELATED QUESTIONS
What would be the molar concentration of human DNA in a human cell?
Distinguish between plasmid DNA and chromosomal DNA.
The technique which involves addition or deletion of genes is ______.
ECoRI is obtained from ______.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Exonuclease performs the function of ____________.
Which molecules are called molecular scissors? Why are they called so?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
