Question

In recombinant DNA technology a plasmid vector must be cleaved by ______.

Options

• the same enzyme that cleaves the donor gene

• modified DNA ligase

• a heated alkaline solution

• four separate enzymes

Answer 1

In recombinant DNA technology a plasmid vector must be cleaved by the same enzyme that cleaves the donor gene.

Explanation:

In recombinant DNA technology, the plasmid vector must be cleaved by the same restriction enzyme as the donor gene. This ensures that the vector and gene have complementary sticky ends, allowing the donor gene to be properly inserted and ligated into the plasmid.