Question

One of the following is not a step involved in recombinant DNA technology:

Options

• cuts made in a DNA molecule with the help of restriction enzymes.

• joining of DNA fragments with the vector.

• cloning of hybrid DNA into the host.

• karyotyping of the host DNA.

Answer 1

Karyotyping of the host DNA

Explanation:

Karyotyping, which studies the number and structure of chromosomes, is not associated with recombinant DNA technology. The actual steps are cutting DNA with restriction enzymes, attaching pieces to vectors, and cloning the recombinant DNA in a host cell for replication and expression.