Advertisements
Advertisements
Question
Give a brief description of the principle of column Chromatography.
Advertisements
Solution
- The principle behind chromatography is a selective distribution of a mixture of organic substances between two phases a stationary phase and a moving phase. The stationary phase can be a solid or liquid, while the moving phase is a liquid or a gas. when the stationary phase is solid, the moving phase is a liquid or gas.
- If the stationary phase is solid, the basis is adsorption, and when it is a liquid, the basis is partition.
- Chromatography is defined as a technique for the separation of a mixture brought about by the differential movement of the individual component through porous medium under the influence of moving solvent.
- In column chromatography, the above principle is carried out in a long glass column.
APPEARS IN
RELATED QUESTIONS
The purity of an organic compound is determined by ______.
A liquid which decomposes at its boiling point can be purified by ______.
Assertion: \[\begin{array}{cc}
\ce{CH - C = CH - COOH}\\|\phantom{.}\\
\phantom{........}\ce{COOC2H5}\end{array}\]
Reason: The principal functional group gets lowest number followed by double bond (or) triple bond.
Give a brief description of the principles of Fractional distillation.
The fragrance of flowers is due to the presence of some steam volatile organic compounds called essential oils. These are generally insoluble in water at room temperature but are miscible with water vapour in vapour phase. A suitable method for the extraction of these oils from the flowers is ______.
If a liquid compound decomposes at its boiling point, which method (s) can you choose for its purification. It is known that the compound is stable at low pressure, steam volatile and insoluble in water.
A liquid with high boiling point decomposes on simple distillation but it can be steam distilled for its purification. Explain how is it possible?
A liquid compound (x) can be purified by steam distillation only if it is ______.
Which statement best explains why organic compounds must be purified?
When is purification of an organic compound usually required?
