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Question
Give a brief description of the principle of column Chromatography.
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Solution
- The principle behind chromatography is a selective distribution of a mixture of organic substances between two phases a stationary phase and a moving phase. The stationary phase can be a solid or liquid, while the moving phase is a liquid or a gas. when the stationary phase is solid, the moving phase is a liquid or gas.
- If the stationary phase is solid, the basis is adsorption, and when it is a liquid, the basis is partition.
- Chromatography is defined as a technique for the separation of a mixture brought about by the differential movement of the individual component through porous medium under the influence of moving solvent.
- In column chromatography, the above principle is carried out in a long glass column.
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