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प्रश्न
Give a brief description of the principle of column Chromatography.
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उत्तर
- The principle behind chromatography is a selective distribution of a mixture of organic substances between two phases a stationary phase and a moving phase. The stationary phase can be a solid or liquid, while the moving phase is a liquid or a gas. when the stationary phase is solid, the moving phase is a liquid or gas.
- If the stationary phase is solid, the basis is adsorption, and when it is a liquid, the basis is partition.
- Chromatography is defined as a technique for the separation of a mixture brought about by the differential movement of the individual component through porous medium under the influence of moving solvent.
- In column chromatography, the above principle is carried out in a long glass column.
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संबंधित प्रश्न
Ortho and para – nitro phenol can be separated by ______.
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A liquid which decomposes at its boiling point can be purified by ______.
Assertion: \[\begin{array}{cc}
\ce{CH - C = CH - COOH}\\|\phantom{.}\\
\phantom{........}\ce{COOC2H5}\end{array}\]
Reason: The principal functional group gets lowest number followed by double bond (or) triple bond.
Explain paper chromatography.
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By mistake, an alcohol (boiling point 97°C) was mixed with a hydrocarbon (boiling point 68°C). Suggest a suitable method to separate the two compounds. Explain the reason for your choice.
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Reason (R): The coloured components of inks migrate at different rates because paper selectively retains different components according to the difference in their partition between the two phases.
A liquid compound (x) can be purified by steam distillation only if it is ______.
Which statement best explains why organic compounds must be purified?
