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प्रश्न
Explain paper chromatography.
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उत्तर
Paper chromatography (PC) is an example of partition chromatography. The same procedure is followed as in thin layer chromatography except that a strip of ‘ paper acts as an adsorbent. This method involves continues differential portioning of components of a mixture between stationary and mobile phases. In paper chromatography, a special quality paper known as chromatography paper is used. This paper act as a stationary phase.
A strip of chromatographic paper spotted at the base with the solution of the mixture is suspended in a suitable solvent which act as the mobile phase. The solvent rises up and flows over the spot. The paper selectively retains different components according to their different partition in the two phases where a chromatogram is developed. The spots of the separated colored compounds are visible at different heights from the position of initial spots . on the chromatogram. The spots of the separated colorless compounds may be observed either under ultraviolent light or by the use of an appropriate spray reagent.
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संबंधित प्रश्न
Explain, why an organic liquid vaporises at a temperature below its boiling point in its steam distillation?
The purity of an organic compound is determined by ______.
A liquid which decomposes at its boiling point can be purified by ______.
Assertion: \[\begin{array}{cc}
\ce{CH - C = CH - COOH}\\|\phantom{.}\\
\phantom{........}\ce{COOC2H5}\end{array}\]
Reason: The principal functional group gets lowest number followed by double bond (or) triple bond.
Give a brief description of the principles of Fractional distillation.
By mistake, an alcohol (boiling point 97°C) was mixed with a hydrocarbon (boiling point 68°C). Suggest a suitable method to separate the two compounds. Explain the reason for your choice.
Assertion (A): Components of a mixture of red and blue inks can be separated by distributing the components between stationary and mobile phases in paper chromatography.
Reason (R): The coloured components of inks migrate at different rates because paper selectively retains different components according to the difference in their partition between the two phases.
A liquid compound (x) can be purified by steam distillation only if it is ______.
Which statement best explains why organic compounds must be purified?
When is purification of an organic compound usually required?
