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प्रश्न
Explain paper chromatography.
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उत्तर
Paper chromatography (PC) is an example of partition chromatography. The same procedure is followed as in thin layer chromatography except that a strip of ‘ paper acts as an adsorbent. This method involves continues differential portioning of components of a mixture between stationary and mobile phases. In paper chromatography, a special quality paper known as chromatography paper is used. This paper act as a stationary phase.
A strip of chromatographic paper spotted at the base with the solution of the mixture is suspended in a suitable solvent which act as the mobile phase. The solvent rises up and flows over the spot. The paper selectively retains different components according to their different partition in the two phases where a chromatogram is developed. The spots of the separated colored compounds are visible at different heights from the position of initial spots . on the chromatogram. The spots of the separated colorless compounds may be observed either under ultraviolent light or by the use of an appropriate spray reagent.
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\phantom{........}\ce{COOC2H5}\end{array}\]
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