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Questions
Explain the process of aminoacylation of tRNA and its role in the process of translation.
How and why is charging of tRNA essential in the process of translation?
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Solution
The process of polymerizing amino acids to create polypeptides is referred to as translation. The nucleotides in the mRNA's sequence determine the order and sequence of amino acids. Peptide bonds, which connect the amino acids, are ultimately hold them together. Energy is needed for peptide bond formation. As a result, during the first phase alone, amino acids are activated in the presence of ATP and connected to their corresponding tRNAs, a process known as charging of tRNA or, to be more precise, aminoacylation of tRNA. It would be energetically advantageous for two of these charged tRNAs to create a peptide bond if they were placed close enough to each other side. A catalyst would speed up the process of peptide bond formation.
RELATED QUESTIONS
Ribosomes are composed of two subunits; the smaller subunit of a ribosome has a binding site for _________ and the larger subunit has two binding sites for two __________.
The three codons which result in the termination of polypeptide chain synthesis are ______.
In an mRNA molecule, untranslated regions (UTRs) are present at ______.
UTRs are the untranslated regions present on ______.
Which of the following statements is correct regarding ribosomes?
Which of the following are correct statements for a tRNA molecule?
- It is an adapter molecule.
- Previously called sRNA (soluble RNA)
- tRNA has a codon loop that has bases complementary to the code,
- it also has an amino acid accepter end to which it binds to amino acids.
- It is non-specific for each amino acid.
Aminoacylation of tRNA also known as ______.
(a) Identify the polarity of x to x’ in the diagram below and mention how many more amino acids are expected to be added to this polypeptide chain.

(b) Mention the codon and anticodon for alanine.
(c) Why are some untranslated sequences of bases seen in mRNA coding for a polypeptide? Where exactly are they present on mRNA?
Codon on mRNA and anticodon on tRNA are ______.
How many different proteins does the ribosome consist of?
