Advertisements
Advertisements
Question
A host cell must be made competent, before it is able to receive an rDNA. Justify.
Advertisements
Solution
Because DNA molecules are hydrophilic, they cannot cross cell membranes. Recombinant DNA must be injected into the cell in order for it to be integrated into the vector or host genome. Making the host cells capable of receiving the rDNA is crucial.
RELATED QUESTIONS
Explain enzyme – replacement therapy to treat adenosine deaminase deficiency. Mention two disadvantages of this procedure.
Write the function of adenosine deaminase enzyme.
Recombination DNA−technology is of great importance in the field of medicine. With the help of a flow chart, show how this technology has been used in preparing genetically engineered human insulins.
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
Give the full form of ELISA. Which disease can be detected using it? Discuss the principle underlying the test.
What is a recombinant DNA vaccine? Give two examples.
The diagram given below represents the schematic structure of proinsulin, which undergoes certain modifications before it becomes a fully functional insulin.
Study the diagram carefully and answer the questions that follow:
- State the change the proinsulin undergoes to become fully functional.
- Name the modern scientific technique used for the production of human insulin.
- How are the two polypeptide chains of the fully functional insulin held together?
Which molecular diagnostic tool relies on the hybridisation of labelled single-stranded DNA to detect specific complementary sequences or mutant alleles?
Which diagnostic technique utilizes antigen-antibody interactions coupled with an enzyme colour reaction, making it well-suited for rapid mass screening?
