Question

Which one of the following is not a component required for Polymerase Chain Reaction (PCR)?

Options

• Template DNA

• Primers

• DNA Ligase

• DNA Polymerase

Answer 1

DNA Ligase

Explanation:

PCR requires a template DNA, short primers and a thermostable DNA polymerase (plus dNTPs and buffer) to amplify target sequences by repeated denaturation–annealing–extension cycles. DNA ligase joins DNA fragments (ligation) and is not required in standard PCR amplification.