Question

Which does not describe a function of the DNA polymerase molecule?

Options

• Recognise the free nucleotide that pairs with the base on the template strand of DNA.

• Read the strand of template DNA and recognise the base there.

• Proofread to ensure that the proper base has been incorporated.

• Make the proper nucleotide to match with the base read on the template strand.

Answer 1

Make the proper nucleotide to match with the base read on the template strand.

Explanation:

DNA polymerase is an enzyme responsible for assembling new DNA strands by linking existing building blocks, but it does not actually create or “make” the nucleotides themselves. Nucleotides (deoxyribonucleoside triphosphates) are synthesised by separate metabolic pathways within the cell and must be present in the surrounding environment for the polymerase to use. The actual functions of DNA polymerase include reading the template strand to identify which base is present, recognising and selecting the correct free-floating complementary nucleotide from the cell’s supply, and proofreading the newly added bases to ensure accuracy and repair any mismatches.