Question

What is the role of topoisomerase I in prokaryotic DNA packaging?

Options

• Maintains the supercoiled state of the nucleoid

• Introduces negative supercoils into DNA

• Synthesises RNA connectors for loop formation

• Binds histone proteins to DNA

Answer 1

Topoisomerase I maintains the supercoiled state of the prokaryotic nucleoid by relaxing positive supercoils. This enzyme is essential for balancing the topological stress generated during DNA replication and transcription, preventing excessive positive supercoiling that would inhibit these vital processes.