Question

What is the role of restriction endonucleases in rDNA technology?

Answer 1

Restriction endonucleases are enzymes that cut DNA at specific sequences. They are important in recombinant DNA (rDNA) technology for:

1. Cutting DNA into smaller pieces.
2. Forming sticky or blunt ends for joining DNA.
3. Helping in gene splicing and inserting genes into vectors like plasmids.