Question

What is the direction of the template strand during transcription?

Options

• 5′→3′

• 3′→5′

• 3′→3′

• 5′→5′

Answer 1

The template strand runs in the 3′→5′ direction during transcription, which allows RNA polymerase to read the template and synthesize mRNA in the 5′→3′ direction. This directional reading is essential for proper base pairing and accurate genetic information transfer.