Question

What is the advantage of a cDNA library?

Answer 1

1. cDNA libraries represent only the expressed genes (exons), excluding introns and non-coding regions, because they are made from mRNA transcripts.
2. They provide insights into gene expression profiles of specific tissues or cells at a given time.
3. The library is useful for isolating genes without introns, which is important for cloning eukaryotic genes into prokaryotic systems.
4. cDNA libraries help in the study of functional genes and are used in gene expression and functional genomics studies.
5. They allow researchers to identify and produce proteins encoded by genes of interest.