Question

What are the three main steps involved in the construction of a transgene?

Answer 1

The three main steps involved in the construction of a transgene are:

1. Isolation of the gene of interest: The desired gene is identified and extracted from the source organism’s DNA.
2. Linking the gene to a suitable vector: The isolated gene is inserted into a vector DNA (such as a plasmid or viral DNA) to facilitate its transfer and integration.
3. Introduction of the gene into the host organism: The recombinant DNA (transgene) is introduced into the host organism’s cells, enabling expression of the foreign gene.